Her brother Prince Mbonisi Zulu on Friday confirmed the news of her death, saying she passed away at a Newcastle hospital.
Misuzulu was officially recognised on Wednesday in terms of the Traditional and Khoi-San Leadership Act.
The late king's will left the throne to his wife Queen Mantfombi Dlamini of KwaKhangelamaNkengane royal palace however, she nominated her first-born son Misuzulu, instead.
Family factions and legal wrangling have cast one of the most secretive royal families in South Africa into the spotlight.
Judge Madondo dismissed with costs the application by Prince Mbonisi Zulu to stop his nephew, Misuzulu kaZwelithini, from being crowned as the Zulu king.
Her application was dismissed with costs by Judge Mjabuliseni Madondo on Wednesday morning.
Two of the late King Goodwill Zwelithini’s daughters are challenging his signature on the will, which opened the way for the late Queen Regent Mantfombi Dlamini Zulu to take the throne.
Two matters are linked with the late King Goodwill Zwelithini’s will, the other matter involves an interdict to prevent an alleged secret coronation of Misuzulu kaZwelithini, who was announced as king.
On Wednesday, Judge Isaac Madondo dismissed Queen Sibongile Winifred Zulu’s application where she asked the court to look into the late King Goodwill Zwelithini’s marriages with his other five wives.
Contained in the papers is an application by the queen, wanting to have her late husband's other marriages declared invalid.
Queen Sibongile Winifred Zulu is the first wife of late Zulu monarch King Goodwill Zwelithini kaBhekuzulu. She married him in a civil union and wants to inherit 50% of the late king's estate.
The late King Goodwill Zwelithini's first wife, Queen Sibongile Winifred Zulu, is asking the court to recognise her civil marriage with the late monarch.
Queen Sibongile Winifred Zulu from the KwaKhethomthandayo royal palace is challenging the matter of her husband’s will, saying that she is the only wife who married the late king in a civil marriage.
The siblings of the late King Goodwill Zwelithini have questioned the legitimacy of their nephew as the rightful successor.
In papers seen by Eyewitness News, King Misuzulu kaZwelithini's lawyers have legally notified Queen Sibongile Dlamini that they want her marriage to the late AmaZulu King Goodwill Zwelithini declared null and void.
The sisters argued in court papers that the late Queen Mantfombi, King Zwelithini’s third wife, had no authority to appoint her son as king of the Zulu nation following the passing of their father.
KZN Premier Sihle Zikalala announced that R66 million has been allocated to the Zulu Royal household sub-programme, meant to provide support to annual traditional and cultural events. Zikalala tabled the province's budget vote on 14 May 2021.
A heated exchange ensued between Buthelezi and Prince Thokozani Zulu after the reading of the late Queen Mantfombi’s will, which recommended her son Prince Misuzulu Zulu become the AmaZulu king.
